[행사/세미나] IPHC Seminar Series / 4. 20
- 지능형정밀헬스케어융합학과
- 조회수721
- 2021-04-15
whole body tracking of single cells via positron emission tomograpy
(Post doctoral Fellow, Kyungoh Jung, Stanford)
In vivo molecular imaging can measure the average kinetics and movement routes of injected cells
through the body. However, owing to non specific accumulation of the contrast agent and its efflux
from the cells, most of these imaging methods inaccurately estimate the distribution of the cells.
Here, we show that single human breast cancer cells loaded with mesoporous s ilica nanoparticles
concentrating the 68Ga radioisotope and injected into immunodeficient mice can be tracked in real
time from the pattern of annihilation photons detected using positron emission tomography, with
respect to anatomical landmarks derived fr om X ray computed tomography. The cells travelled at
an average velocity of 50 mm s 1 and arrested in the lungs 2 3 s after tail vein injection into the
mice, which is consistent with the blood flow rate. Single cell tracking could be used to determine
the kinetics of cell trafficking and arrest during the earliest phase of the metastatic cascade, the
trafficking of immune cells during cancer immunotherapy and the distribution of cells after
transplantation.