YoungWon Koo(Senior year of Biomechatronic Eng.)'s research paper was published in Biofabrication
- Biotech.
- Hit2109
- 2016-05-24
YoungWon Koo(Senior year of Biomechatronic Eng.)'s research paper
was published in Biofabrication
YoungWon Koo(Senior year of Biomechatronic Eng.) "New strategy for enhancing in situ cell viability of cell-printing process via piezoelectric transducer-assisted three-dimensional printing."
Tissue engineering has become one of the great applications of three-dimensional cell printing because of the possibility of fabricating complex cell-laden scaffolds.
Three typical methods (inkjet, micro-extrusion, and laser-assisted bio-printing) have been used to fabricate structures. Of these, micro-extrusion is a comparatively easymethod, but has some drawbacks such as low in situ cell viability after fabricating cell-laden structures because of the highwall shear stress in micro-sized nozzles.
To overcome this shortcoming,we suggest an innovative cell printingmethod, which is assisted by a piezoelectric transducer (PZT). The PZT assistance in the dispensing process enhances the printing efficiency and cell viability by decreasing thewall shear stress within a nozzle because the PZT effect can lower the shear viscosity of the bioink viamicro-scale vibration.
In this study, 5 wt% cell-laden alginatewas used as a bioink, and various PZT conditions (frequencies up to ∼400 Hz and amplitudes up to∼40.5 μm) were simultaneously applied to the cell-printing process to examine the effectiveness of the PZT. The PZT-assisted cell-printing method was found to be highly effective in direct cell printing and could achieve cell-laden structureswith high in situ cell
viability.
...
The cell damage that occurs during the cell-printing process is primarily the result of the wall shear stress in the narrow printing nozzle. An innovative method of enhancing the initial cell viability was proposed using a direct cell printing method assisted by a PZT system, which could significantly reduce the wall shear stress of the bioink within the printing nozzle. By manipulating various PZT conditions (frequency and amplitude), we could successfully attain an optimal processing condition, which allowed a relatively high initial cell viability for the printed cell-laden struts.
Based on the results, we can confirm that the method is one solution for increasing the in situ cell viability in the cell-printing process. Furthermore, the ability to construct a highly porous 3D structure with an appropriate thickness could demonstrate that the cellprinting method may have substantial potential for the successful development of cell-embedded 3D porous structures.